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(A) ANS fluorescence intensity (Fmax) profiles of various proteins (2.0 μM) in ice, at −13°C, as a function of the starting ANS concentration in solution: ALD (•), YADH (◂), LDH (♦), BSA (▴), LADH (▾), α-amylase (▸), β-gal (
), and azurin WT (▪). Other conditions are as in Fig. 1. (B) The same ANS fluorescence profiles of Fig. 4 A normalized by the protein molecular weight (Table 2).
