TABLE 2.

List of ANS fluorescence maxima (λ_max) and minimum number of binding sites (n) for proteins in ice at −13°C

Protein	λmax(nm)	n	Mw · 10−3	10−5 · n/Mw	Rel. area*
Monomers
Azurin WT	480	1.6	14	11.4	2.5
BSA	465	>25.0	67	>37.3	4.9
α-amylase	491	>14.4	45	>21.5	2.2
β-gal	488	9.7	115	8.4	1.0
Dimers
LADH	475	>21.2	80	>26.5	3.5
Tetramers
YADH	486	>44.4	141	>28.3	3.3
ALD	475	>44.8	158	>31.5	2.9
LDH	470	37.6	130.5	28.8	4.7

^*Relative area under the plots of Fig. 4 B.