Real-time tet promoter activity from 10 single cells induced at (A), 200 ng/ml aTc, (B), 400 ng/ml aTc, and (C), 1 μg/ml aTc. Error bars represent uncertainties in the fit parameters extracted from the autocorrelation function. (D) Average profiles of ms2-RNA concentration from 10 single cells. No induction (light gray), 200 ng/ml aTc (gray), 400 ng/ml aTc (dark gray), and 1 μg/ml aTc (black). Cells were immobilized on polylysine-coated surfaces. Error bars represent the standard deviations from the ms2-RNA concentration distributions across a population of 10 cells at a given time point.