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. 2006 Feb 3;90(9):3315–3321. doi: 10.1529/biophysj.105.073353

FIGURE 5.

FIGURE 5

Efflux-mediated inducible transcription. (A) Concentration profiles of ms2-RNA transcripts from an inducible tet promoter in a Frag1A mutant cell (ΔacrAB) without aTc (shaded) and with 400 ng/ml aTc (solid). Wild-type Frag 1B cell with 400 ng/ml (dark shaded). (B) Tet promoter activity with sequential inductions in four nondividing wild-type cells (Frag1B). Cells were first induced with 100 ng/ml aTc at time zero followed by a second induction with 1 μg/ml aTc at time 60 min after first induction. Arrows indicate times of induction. Error bars represent uncertainties in the fit parameters extracted from the autocorrelation function.