TABLE 3.
Experimental conditions of the intracellular titrations and relevant parameters obtained by fitting the ratiometric curves to Eq. 4 with n = 1
| Protein | Setup | Cell line | T (°C) | Confocal aperture* | Rf | pK′ |
|---|---|---|---|---|---|---|
| E2GFP | Ex488:458 | CHO | 23 | 3 | 12.6 ± 1.7 | 6.99 ± 0.04 |
| E2GFP | Ex488:458 | CHO | 23 | 1 | 12.5 ± 1.5 | 7.01 ± 0.04 |
| E2GFP | Ex488:458 | CHO | 37 | 3 | 10.2 ± 1.2 | 6.89 ± 0.04 |
| E2GFP | Ex488:458 | CHO | 37 | 1 | 10.4 ± 0.9 | 6.91 ± 0.04 |
| Tat-E2GFP | Ex488:458 | U-2 OS | 37 | 3 | 14.2 ± 3.4 | 6.83 ± 0.06 |
| Tat-E2GFP | Ex488:458 | U-2 OS | 37 | 1 | 14.5 ± 4.5 | 6.91 ± 0.09 |
| E2GFP | Ex488:406 | CHO | 37 | 3 | 11.0 ± 1.2 | 7.37 ± 0.07 |
| E2GFP | Em515:475 | CHO | 37 | 3 | 5.4 ± 0.2 | 7.48 ± 0.05 |
| Tat-E2GFP | Em515:475 | U-2 OS | 37 | 3 | 6.3 ± 0.3 | 7.21 ± 0.05 |
*
Airy units (1 airy = 81.44 μm for our setup).