TABLE 1.
Oligonucleotides used for PCR of the upstream and downstream fragments used in arcA knockout constructsa
Oligonucleotide | Sequence (5′-3′) |
---|---|
arcA-up1 | GCTCTAGAGGCCTCGACCCCCATCTTTAACGGG TAGTTAAACA |
arcA-up2 | GGGGTACCGGCCATGCGGGCCCTGTGCAACATT ATTGTAACAA |
arcA-down1 | GCTCTAGAGGCGCGCCAGTGATTGTTCTACAT AAAAATT |
arcA-down2 | GGGGTACCGCGGCCGCTGTATTCTTTACGTGA TAGTAAT |
Restriction enzyme sites used for cloning are indicated by boldface type.