TABLE 2.
Oligonucleotides for PCR of the arcA gene
Type of primer (plasmid) | H. influenzae arcA oligonucleotide (5′-3′)a | E. coli arcA oligonucleotide (5′-3′)a |
---|---|---|
5′ (pJRD215) | AACCCAAGCTTATGACTACTCCAAAAATTCTC | AACCCAAGCTTATGCAGACCCCGCACATT |
3′ (pJRD215) | ACATGCCATGGTTATTCAATATCTCCACAAAAAC | ACATGCCATGGTTAATCTTCCAGATCACCG |
5′ (pMTL22) | GCTCTAGAATGACTACTCCAAAAA | GCTCTAGAATGCAGACCCCGCACA |
3′ (pMTL22) | CGGCTAGCCATTGCCTTTCCTCCTTATTCAATATCTCCAC | CGGCTAGCCATTGCCTTTCCTCCTTAATCTTCCAGATCA |
Restriction enzyme sites used for cloning are indicated by boldface type. Underlined nucleotides were added to keep the arcA gene in frame with upstream genomic sequences.