TABLE 2.
Hemolytic activity of encapsulated GAS strains used in this study
| Strain | Mean % erythrocyte lysis ± SEM
|
|
|---|---|---|
| SLO activitya | SLS activityb | |
| 950771 | 48 ± 2.8 | 57 ± 9.2 |
| 950771SmSLO | <1 ± 1.9 | 56 ± 5.1 |
| 950771SLS | 48 ± 6.6 | 2.7 ± 0.9 |
a
A 1:10 dilution of late-exponential-phase culture supernatant was used to lyse a standardized sheep erythrocyte preparation. In all strains, activity was completely inhibited by the addition of cholesterol to a final concentration of 17 μg/ml (data not shown). Values represent means from at least two experiments.
b
A 1:10 dilution of late-stationary-phase cell extract was used to lyse a standardized sheep erythrocyte preparation. In all strains, activity was unaffected by the addition of cholesterol to a final concentration of 17 μg/ml (data not shown). Values represent means from at least two experiments.