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RT-PCR analysis of cytokine mRNA expression in BALB/c mice infected with P. marneffei. Mice were injected i.v. with 3 × 105 conidia. At the indicated times, spleens and livers were excised and processed for evaluation of cytokine gene expression by RT-PCR. Lanes C, control mRNA from three uninfected wild-type mice; lanes N, no DNA was added to the amplification mixture during PCR.
