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RT-PCR analysis of cytokine mRNA in the spleens and livers of wild-type and GKO BALB/c mice infected with P. marneffei. Mice were injected i.v. with 3 × 105 conidia and sacrificed at 7 and 14 days after infection. Spleens and livers were recovered and processed for evaluation of cytokine gene expression by RT-PCR. Lanes C, control mRNA from uninfected wild-type mice; lanes N, no DNA was added to the amplification mixture during PCR.
