FIG. 1.

Formation of TRAP-positive multinucleated cells in the coculture system treated with whole-cell sonicates and LOS from T. denticola. Mouse bone marrow and calvaria cells were cocultured to confluence and treated with T. denticola sonicates (10 μg/ml) or LOS (0.1 to 10 μg/ml) for an additional 4 days. The cells were then stained for TRAP. The TRAP-positive multinucleated cells containing more than three nuclei were counted as osteoclasts. The data are the means ± standard errors for four cultures. An asterisk indicates that the P value is <0.05 for a comparison with the results for the nontreated cultures.