FIG. 3.

Effect of polymyxin B on the ODF and OPG mRNAs induced by T. denticola LOS. After cocultures were treated with LOS (10 μg/ml) in the presence or absence of 10 μg of polymyxin B per ml (Poly10) or 50 μg of polymyxin B per ml (Poly50) for 4 days, the ODF, OPG, and β-actin mRNA levels were examined by RT-PCR (A). The signals in the RT-PCR were quantified and normalized to β-actin mRNA expression by using an image analyzer (B). The experiments were repeated three times, and similar results were obtained in all experiments.