FIG. 5.

Effect of indomethacin on osteoclastogenesis and the expression of ODF and OPG mRNAs modulated by T. denticola LOS. Cocultures were simultaneously treated with LOS (10 μg/ml) with or without indomethacin (Ind) (1 μM) for 4 days. The cells were then stained for TRAP to count the number of osteoclasts. The data are means ± standard errors for three cultures (A). The RNA was isolated from the cultured cells, and the ODF, OPG, and β-actin mRNA levels were analyzed by RT-PCR (B). The RT-PCR signals shown were quantified and normalized to the β-actin mRNA expression by using an image analyzer (C). Representative results of three experiments that yielded similar results are shown. An asterisk indicates that the P value is <0.05 for a comparison with the results for the LOS-treated cultures.