FIG. 6.

Expression of MMP and TIMP mRNAs in mouse calvaria-derived osteoblastic cells treated with T. denticola LOS. After the osteoblastic cells were treated with T. denticola LOS (10 μg/ml) in the absence (A) or in the presence (C) of polymyxin B (Poly) (50 μg/ml) for 48 h, the RNA was isolated from the cells, and the MMP and TIMP mRNA levels were analyzed by RT-PCR. Representative results of three experiments that yielded similar results are shown. The RT-PCR signals shown in panels A and C were quantified and normalized to β-actin mRNA expression by using an image analyzer (B and D).