FIG. 6.

Lack of complementation of S. flexneri mutations. (A) Protein contents of cultures of S. flexneri wild-type (M90T), ipgC (RM97), ipgC carrying plac sycB (pBF15), and ipaB (RM221) strains grown for 4 h at 37°C were analyzed by Western blotting with monoclonal anti-IpaB antibody. (B) Protein contents of cultures of the S. flexneri ipaB strain, wild-type strain, ipaB ipgC (SF1068) strain, and ipaB ipgC sycB⁺ strain carrying pBF25 grown for 4 h at 37°C were analyzed by Western blotting with polyclonal anti-IpaH antibody. (C) Protein contents of culture supernatants of the S. flexneri wild-type strain, ipaB strain, and ipaB sycB⁺ yspB⁺ strain carrying pBF26 grown for 4 h at 37°C were determined by staining with Coomassie blue. (D) Protein contents of supernatants of the S. flexneri ipaC (RM81) strain and ipaC sycB⁺ yspC⁺ strain carrying pBF29 after Congo red induction were analyzed by Western blotting with polyclonal anti-IpaB antibody. WT, wild type; MW, molecular weights in thousands.