TABLE 3.

Ag85-specific Th1-type cytokine secretion in BALB/c and C57BL/6 mice vaccinated with DNA encoding the three Ag85 components

Mice	Vaccination	Mean IL-2 (cpm) ± SDa			Mean IFN-γ (pg/mL) ± SDb
		Ag85A DNA	Ag85B DNA	Ag85C DNA	Ag85A DNA	Ag85B DNA	Ag85C DNA
BALB/c	Medium	2,595 ± 512	3,752 ± 1,118	2,939 ± 663	<50	<50	<50
	Ag85Ac	22,090 ± 9,898	5,784 ± 1,118	4,005 ± 1,146	1,092 ± 435	293 ± 80	142 ± 104
	Ag85B	4,742 ± 1,657	8,519 ± 2,972	3,202 ± 328	266 ± 104	472 ± 233	142 ± 90
	Ag85C	7,301 ± 2,780	4,306 ± 770	10,307 ± 1,809	523 ± 163	482 ± 122	396 ± 99
	Ag85	13,237 ± 4,041	7,392 ± 1,329	7,481 ± 1,560	779 ± 78	699 ± 284	378 ± 93
C57BL/6	Medium	1,848 ± 230	2,116 ± 406	1,897 ± 232	<50	<50	<50
	Ag85A	21,308 ± 1,568	16,300 ± 4,764	2,788 ± 593	2,737 ± 226	1,517 ± 462	<50
	Ag85B	7,714 ± 2,051	13,319 ± 4,765	2,571 ± 271	1,018 ± 432	2,300 ± 711	287 ± 191
	Ag85C	7,343 ± 3,432	6,072 ± 1,730	6,202 ± 2373	1,170 ± 661	612 ± 136	2,392 ± 713
	Ag85	16,487 ± 4,687	12,860 ± 4,297	4,252 ± 755	2,300 ± 700	1,926 ± 829	934 ± 390

^aMean IL-2 levels measured in 24-h culture supernatant of spleen cells from mice vaccinated with DNA and unstimulated (medium) or restimulated in vitro with Ag85 components. Four mice were tested individually in each group. Data from corresponding DNA and antigen pairs are given in bold.

^bMean IFN-γ levels measured in 72-h culture supernatant of spleen cells from mice vaccinated with DNA and unstimulated (medium) or restimulated in vitro with Ag85 components. Four mice were tested individually in each group.

^cAntigen used for in vitro restimulation (5 μg/ml).