TABLE 3.

Expression of CD44 (CY 5) on live and early apoptotic activated CD4⁺ cells^a

Animal no.	M. bovis PPD stimulated		PWM stimulated
	Annexin V− 7AAD−	Annexin V+ 7AADdim/−	Annexin V− 7AAD−	Annexin V+ 7AADdim/−
408	356.1	172.4	286.4	7.6
410	322.7	124.5	294.4	7.3
426	188.8	143.9	207.8	5.5
Mean ± SEMb	289.2 ± 51.1*	146.9 ± 13.9	262.9 ± 27.6**	6.8 ± 0.7

^aThe geometric mean fluorescence (CY 5) intensity of CD44 was determined for CD4⁺ (i.e., PE⁺) and either Annexin V⁻ 7AAD⁻ (i.e., live) or Annexin V⁺ 7AAD^dim/− (i.e., early apoptotic) cells. Animals 408, 410, and 426 were aerosol infected (10⁵ CFU) with M. bovis. Responses to PWM were from PBMC obtained from three noninfected animals.

^b*, differs (P = 0.05) from responses of PPD-stimulated, Annexin V⁺ 7AAD^dim/− cells; **, differs (P = 0.002) from responses of PWM-stimulated, Annexin V⁺ 7AAD^dim/− cells.