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(a) Nuclear fragmentation ELISA from THP-1 cells 3 days after infection with avirulent BCG at an infectious dose of 5 or 10 bacilli per macrophage. Data are means plus standard errors of four experiments. ∗, statistically significant difference between groups (P < 0.05). (b) Annexin V-FITC staining of THP-1 cells uninfected (gray) or infected with BCG (black overlay). At 5 days postinfection, 72% of cells were apoptotic.
