FIG. 3.
DAPI nuclear staining of uninfected THP-1 cells (a), THP-1 cells infected with BCG for 5 days (b), and uninfected THP-1 cells treated with 100 U of rTNF/ml (c). Arrows, condensed nuclei of apoptotic cells. (d) Annexin V-FITC staining of uninfected (gray) and rTNF-treated (black overlay) THP-1 cells. (e) Nuclear fragmentation ELISA of THP-1 cells unstimulated (Un) or treated with 100 U of rTNF/ml for 3 days. Data are means plus standard errors of four experiments (P = 0.530 by Student's t test).