FIG. 2.

Binding of T. cruzi metacyclic-stage surface molecule gp82 to gastric mucin. (A) gp82, either purified from parasite extracts or as a recombinant protein containing the entire gp82 peptide sequence (J18) or the C-terminal domain of gp82 (J18b), at 10 μg/ml, was added to microtiter plates coated with porcine gastric mucin. (B) Increasing concentrations of the T. cruzi surface molecule gp90, gp35/50, or gp82, as well as the recombinant protein J18, were added to wells in microtiter plates coated with gastric mucin. (C) Increasing doses of J18b were added to plates containing adherent HeLa cells. In all ELISAs, after washes, the plates were sequentially incubated with anti-gp82 antibody, anti-gp90 MAb 1G7, or anti-gp35/50 MAb 10D8 and anti-mouse immunoglobulin conjugated to peroxidase. The bound enzyme was revealed by using o-phenylenediamine. The values are the means ± standard deviations of triplicate samples. O.D.₄₉₂, optical density at 492 nm.