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Immunogold labeling of the wild-type strain (A and C) and the lpeA mutant (B and D) of L. monocytogenes, using as control a polyclonal rabbit anti-Listeria antiserum (A and B) and a monoclonal anti-ScaA from S. gordonii directed against LpeA (C and D). Both strains are strongly labeled by the polyclonal anti-Listeria serum. Only the wild-type bacteria were labeled by the MAb anti-ScaA, contrasting with the absence of labeling of the lpeA mutant.
