TABLE 3.

Characterization of T. denticola GGT^a

Treatment	Conditions	Enzyme activity (% of control)
		GGT	Cystalysin
Control	Buffer only	100.0 ± 5.6	100.0 ± 5.6
Proteinase inhibitor	100 μg/ml
TLCK	2 mM	5.8 ± 0.5	96.8 ± 10.2
PMSF	2 mM	95.8 ± 5.8	98.4 ± 8.8
Benzamidine	2 mM	98.5 ± 8.9	95.5 ± 6.7
Thiol compound
2-ME	6 mM	255.1 ± 16.8	189.5 ± 12.6
l-Cysteine	6 mM	155.9 ± 13.7	ND
Dithiothreitol	6 mM	219.8 ± 18.9	168.9 ± 8.6
Proteinase
Proteinase K	100 μg/ml	4.9 ± 0.6	91.8 ± 7.6
Pronase	100 μg/ml	15.4 ± 1.8	95.4 ± 8.9
Salt
NaCL	600 mM	105.8 ± 9.6	101.5 ± 12.4
KCL	600 mM	155.6 ± 8.9	98.4 ± 10.2
Heating	56°C	20.5 ± 2.1	92.5 ± 8.9
Freezing	−20°C	98.5 ± 6.8	100.5 ± 11.9

^aPurified recombinant GGT and cystalysin of T. denticola were compared with regard to their sensitivities to various treatments. Significant differences between the two enzymes in their sensitivities to TLCK, proteinase, heating, and KCl treatment are shown. ND, not determined.