TABLE 3.
Treatment | Conditions | Enzyme activity (% of control)
|
|
---|---|---|---|
GGT | Cystalysin | ||
Control | Buffer only | 100.0 ± 5.6 | 100.0 ± 5.6 |
Proteinase inhibitor | 100 μg/ml | ||
TLCK | 2 mM | 5.8 ± 0.5 | 96.8 ± 10.2 |
PMSF | 2 mM | 95.8 ± 5.8 | 98.4 ± 8.8 |
Benzamidine | 2 mM | 98.5 ± 8.9 | 95.5 ± 6.7 |
Thiol compound | |||
2-ME | 6 mM | 255.1 ± 16.8 | 189.5 ± 12.6 |
l-Cysteine | 6 mM | 155.9 ± 13.7 | ND |
Dithiothreitol | 6 mM | 219.8 ± 18.9 | 168.9 ± 8.6 |
Proteinase | |||
Proteinase K | 100 μg/ml | 4.9 ± 0.6 | 91.8 ± 7.6 |
Pronase | 100 μg/ml | 15.4 ± 1.8 | 95.4 ± 8.9 |
Salt | |||
NaCL | 600 mM | 105.8 ± 9.6 | 101.5 ± 12.4 |
KCL | 600 mM | 155.6 ± 8.9 | 98.4 ± 10.2 |
Heating | 56°C | 20.5 ± 2.1 | 92.5 ± 8.9 |
Freezing | −20°C | 98.5 ± 6.8 | 100.5 ± 11.9 |
Purified recombinant GGT and cystalysin of T. denticola were compared with regard to their sensitivities to various treatments. Significant differences between the two enzymes in their sensitivities to TLCK, proteinase, heating, and KCl treatment are shown. ND, not determined.