Identification of a new UDP-glucosyltransferase activity. Ultrasonic extracts from different Legionella species were incubated in the presence of HeLa cell extract and [14C]UDP-glucose. After this, the samples were subjected to SDS-PAGE, dried, and exposed to X-ray film. Lane 1, L. pneumophila Philadelphia I; lane 2, L. pneumophila 130b; lane 3, L. pneumophila ATCC 33823; lane 4, L. pneumophila ATCC 35096; lane 5, L. longbeachae ATCC 33462; lane 6, L. gormanii ATCC 33297; lane 7, L. steigerwaltii ATCC 35302; lane 8, L. pneumophila Philadelphia I without eukaryotic substrate; lane 9, HeLa cell extract without bacterial extract. The data represent data obtained in several experiments.