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. 2003 Jan;71(1):343–353. doi: 10.1128/IAI.71.1.343-353.2003

FIG. 3.

FIG. 3.

Autoradiogram of an 8.0% nondenaturing polyacrylamide gel showing a gel shift assay for purified SarT protein with a sarU promoter fragment. Purified SarT protein was allowed to bind a 148-bp radiolabeled sarU promoter fragment. Lanes 1 to 9, mobility of the 148-bp DNA fragment with 0, 0.2, 0.3, 0.5, 0.75, 1.0, 2.0, 2.5, and 3.0 μg of purified SarT protein, respectively; lanes 10 and 11, mobility of the same fragment in the presence of 1.0 μg of the purified protein and a 40-fold excess (molar ratio) of unlabeled 148-bp sarU fragment and a 273-bp nonspecific DNA fragment, respectively.