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. 2003 Jan;71(1):187–195. doi: 10.1128/IAI.71.1.187-195.2003

FIG. 4.

FIG. 4.

Phylogenetic tree of the 28-kDa OMP locus spanning orthologous and paralogous genes 14 to 19. Protein-coding sequences that encode 28-kDa OMPs from all genes depicted in Fig. 3 were translated and used to construct the phylogenetic tree. Sequences from one isolate each (Arkansas, Jax, and St. Vincent) were used to prepare the tree with the GCG programs Pileup, Distance, and Growtree (7). An OMP, p44-13, a sequence of Anaplasma phagocytophila (GenBank accession no. AF414592), was used as an outgroup to root the tree. Orthologous genes 15, 15a, and 17 clustered on the same branch of the tree, while genes 14, 16, 18, and 19 grouped on separate branches. Symbols I, II, and III in the figure represent genes from isolates of Groups I, II, and III, respectively.