Skip to main content
. 2003 Jan;71(1):187–195. doi: 10.1128/IAI.71.1.187-195.2003

FIG. 5.

FIG. 5.

Verification of the mapped 28-kDa locus from Group II and III isolates. To verify the organization of genes, PCR primers were designed to amplify predicted size overlapping fragments. (A) The primers were designed from the variable regions of coding sequences, and the sizes of the predicted amplicons were estimated. Segments A and B verify the location of genes 14 and 15 for Group II isolates. Segments C and G verify the loss of gene 18 in Group II and III isolates. The gain of gene 15a in Group III isolates was verified by amplifying segments D, E, and F. (B) Amplification data for two isolates each (Group II, Wakulla and St. Vincent; Group III, Liberty and Jax) are presented. The predicted amplicons were detected in the PCR products.