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. Author manuscript; available in PMC: 2006 Apr 7.
Published in final edited form as: J Am Chem Soc. 2004 Dec 1;126(47):15392–15393. doi: 10.1021/ja0441716

Figure 1.

Figure 1

(A) Fluorescence response of 5 μM PF1 to 100 μM H2O2. The dotted and solid line spectra were recorded before and after H2O2 addition, respectively. Spectra were acquired in 20 mM HEPES, pH 7 (λexc = 450 nm). (B) Fluorescence responses of 5 μM PF1 to various ROS (10 mM O2−, 100 μM for all other ROS). •OH and •OtBu were generated by reaction of Fe2+ with H2O2 or TBHP, respectively. NO was delivered using S-nitrosocysteine (SNOC). Spectra were acquired in 20 mM HEPES, pH 7, and all data were obtained after incubation with the appropriate ROS at 25 °C for 1 h. Collected emission was integrated between 460 and 700 nm (λexc = 450 nm).