Figure 4.

HR and Mildew Resistance Mediated by RPW8 Exhibit Similar Environmental Sensitivity as SHL in S24.

(A) Four-week-old S24 plants grown on MS-agar plates were inoculated with E. cichoracearum UCSC1 and transplanted immediately to perlite in Magenta jars under standard SHL-permissive conditions (S), in low light (∼14 μmol·m⁻²·s⁻¹) (LL), or in high humidity (RH ≥ 96%) (HH). Inoculated leaves were collected at 48 h after inoculation and stained with trypan blue to visualize cell death and fungal structure. The micrographs at bottom were taken with a light microscope at ×400 magnification. Arrows indicate fungal hyphae.

(B) Four-week-old S5 and Col-0 plants were inoculated with E. cichoracearum UCSC1. After inoculation, plants were shifted to normal conditions (see Methods) (N), low light (∼14 μmol·m⁻²·s⁻¹) (LL), or high humidity (RH ≥ 85%) (HH). Photographs were taken at 8 days after inoculation.