Figure 2.

Purification of AtPGIP1 and AtPGIP2.

(A) Inhibitory activity of purified AtPGIP1 and AtPGIP2. Desalted total protein extracts from 1-5E1 (squares) and 2-4A3 (diamonds) plants, overexpressing AtPGIP1 and AtPGIP2, respectively, and from untransformed plants (triangles) were subjected to chromatography on a cation-exchange column (SP-Sepharose). Inhibitory activity of the collected fractions was tested against PG of Botrytis.

(B) SDS-PAGE of AtPGIPs purified from overexpressing transgenic plants (left) and immunodetection using a polyclonal antibody against bean PGIP (right). Lanes 1, molecular mass markers; lanes 2, PGIP purified from bean pods; lanes 3, AtPGIP1; lanes 4, AtPGIP2. The sizes of the marker bands shown in lanes 1 are indicated at right.