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. 2003 Jan;15(1):93–106. doi: 10.1105/tpc.005165

Figure 7.

Figure 7.

Accumulation of PGIP Activity in Response to OGs, Cold, and MeJA.

Inhibition of Botrytis (striped bars) and C. gloeosporioides (open bars) endo-PGs by protein extracts from seedlings treated with OGs (A), low temperature (B), or MeJA (C).

(A) Seedlings were treated with OGs (100 μg/mL) and harvested at the indicated times. Bars represent the average inhibition of PG activity ± sd of three independent experiments, using 8 μg of total protein. The comparable ability to inhibit these two enzymes indicates that the induced PGIP is represented mainly by AtPGIP1. No inhibitory activity was detected in untreated control plants.

(B) Seedlings were incubated at 4°C and harvested at the indicated times. Bars represent the average inhibition of PG activity ± sd of three independent experiments, using 3 μg of total protein. The comparable ability to inhibit these two enzymes indicates that the induced PGIP is represented mainly by AtPGIP1. No inhibitory activity was detected in untreated control plants.

(C) Seedlings were treated with 50 μM MeJA and harvested at the indicated times. Bars represent the average inhibition of PG activity ± sd of three independent experiments, using 3 μg of total protein. Superimposed black bars indicate the activity against Botrytis PG detected in control plants. The lower inhibition of C. gloeosporioides PG indicates that the induced PGIP is represented mainly by AtPGIP2.