Abstract
To investigate the role of epithelial-mesenchymal interactions on intestinal maturation, we cultured embryonic epithelial cells in several experimental conditions. Microexplants of 14-15 days fetal rat intestinal endoderm, separated from the mesenchyme by collagenase, were seeded on dishes coated with different extracellular matrix components (collagens I, III, IV, fibronectin, laminin) or on confluent monolayers of intestinal mesenchymal cells or of fetal skin fibroblasts. Only small variations in the attachment or spreading of the endodermal cells could be observed when they were cultured on the different substrata and their survival never exceeded one week. When cocultured with intestinal or skin fibroblasts, however, endodermal cells grew, formed a monolayer, survival time was prolonged up to two to three weeks, and differentiation occurred. This differentiation was assessed by cell polarisation, morphological maturation of apical brush borders, synthesis of microvillar digestive enzymes and of extracellular matrix molecules seen immunocytochemically. Finally, glucocorticoids which are known to stimulate or induce brush border enzymes, accelerated the morphological and enzymatic maturation only in the cocultures.
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- Bissell M. J., Hall H. G., Parry G. How does the extracellular matrix direct gene expression? J Theor Biol. 1982 Nov 7;99(1):31–68. doi: 10.1016/0022-5193(82)90388-5. [DOI] [PubMed] [Google Scholar]
- Dow K. E., Sabry K., Smith B. T. Evidence for epithelial-mesenchymal interactions mediating glucocorticoid effects in developing chick liver. Fibroblast-hepatocyte factor. Cell Tissue Res. 1983;231(1):83–91. doi: 10.1007/BF00215776. [DOI] [PubMed] [Google Scholar]
- Haffen K., Kedinger M., Simon-Assmann P. Mesenchyme-dependent differentiation of epithelial progenitor cells in the gut. J Pediatr Gastroenterol Nutr. 1987 Jan-Feb;6(1):14–23. doi: 10.1097/00005176-198701000-00005. [DOI] [PubMed] [Google Scholar]
- Hauri H. P., Quaroni A., Isselbacher K. J. Monoclonal antibodies to sucrase/isomaltase: probes for the study of postnatal development and biogenesis of the intestinal microvillus membrane. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6629–6633. doi: 10.1073/pnas.77.11.6629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kédinger M., Simon-Assmann P., Alexandre E., Haffen K. Importance of a fibroblastic support for in vitro differentiation of intestinal endodermal cells and for their response to glucocorticoids. Cell Differ. 1987 Mar;20(2-3):171–182. doi: 10.1016/0045-6039(87)90431-3. [DOI] [PubMed] [Google Scholar]
- Levine J. F., Stockdale F. E. Cell-cell interactions promote mammary epithelial cell differentiation. J Cell Biol. 1985 May;100(5):1415–1422. doi: 10.1083/jcb.100.5.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mathan M., Hermos J. A., Trier J. S. Structural features of the epithelio-mesenchymal interface of rat duodenal mucosa during development. J Cell Biol. 1972 Mar;52(3):577–588. doi: 10.1083/jcb.52.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quaroni A. Development of fetal rat intestine in organ and monolayer culture. J Cell Biol. 1985 May;100(5):1611–1622. doi: 10.1083/jcb.100.5.1611. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quaroni A., May R. J. Establishment and characterizaton of intestinal epithelial cell cultures. Methods Cell Biol. 1980;21B:403–427. [PubMed] [Google Scholar]
- Quaroni A. Use of monoclonal antibodies in the study of intestinal structure and function. Ciba Found Symp. 1983;95:113–131. doi: 10.1002/9780470720769.ch8. [DOI] [PubMed] [Google Scholar]
- Simon-Assmann P., Kedinger M., Haffen K. Immunocytochemical localization of extracellular-matrix proteins in relation to rat intestinal morphogenesis. Differentiation. 1986;32(1):59–66. doi: 10.1111/j.1432-0436.1986.tb00556.x. [DOI] [PubMed] [Google Scholar]