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. Author manuscript; available in PMC: 2006 Aug 1.
Published in final edited form as: J Med Microbiol. 2005 Aug;54(Pt 8):785–794. doi: 10.1099/jmm.0.46077-0

Fig. 3.

Fig. 3

Validation of cell-specific markers for HPLFs and GMSM-K cells. HPLFs and GMSM-K cells were grown separately in Medium 199 for 24 h on chamber slides. Slides were treated with either Ab-1 or mouse anti-SV40 T-antigen mAb (diluted 1 : 1000) followed by Alexa Fluor 488 goat anti-mouse IgG (diluted 1 : 1000). GMSM-K cells that were treated with Ab-1 mAb were also labelled with DAPI (GMSM-K inset). HPLFs that were treated with SV40 T-antigen mAb were also labelled with Texas red-X– phalloidin (HPLF inset). All slides were examined using a fluorescence microscope and images recorded with a digital camera. Magnification ×60.