Figure 1.

Alignment of the Predicted Protein Sequences of Stisa1, Stisa2, and Stisa3 with the Isoamylase from P. amyloderamosa.

Protein sequences were aligned using CLUSTAL W and FUGUE. Regions of β-strand in P. amyloderamosa isoamylase (Paisa) are indicated (b) below the alignment, regions of α-helix are indicated (a) below the alignment, and regions of 3/10 helix are indicated (3) below the alignment. For clarity, the regions of β-strand and the loops between these and the regions of α-helix in the (αβ)₈ barrel structure are labeled in red and purple, respectively. The eight residues absolutely conserved in all active members of the α-amylase superfamily are indicated by green asterisks. The predicted first amino acids in the mature peptides of Stisa1, Stisa2, and Stisa3 are boxed in purple.