Table 1.
Activity of Isoamylase Isoforms from Potato on Different Glucan Substrates
| Glucan
|
||||||
|---|---|---|---|---|---|---|
| Isoamylase | Amylopectin | β-Limit Dextrin | Pullulan | Phytoglycogen | Potato Starch (Boiled) | Potato Starch (Not Boiled) |
| Stisa1 | 5.871 ± 0.461 | 1.072 ± 0.081 | 0.222 ± 0.102 | 0.747 ± 0.078 | 0.268 ± 0.163 | 0.013 ± 0.078 |
| Stisa2 | 0.069 ± 0.047 | 0.020 ± 0.207 | 0.251 ± 0.144 | 0.092 ± 0.148 | 0.346 ± 0.245 | 0.010 ± 0.029 |
| Stisa3 | 4.328 ± 1.090 | 74.135 ± 1.590 | 0.310 ± 0.235 | 1.345 ± 0.344 | 6.257 ± 0.479 | 0.143 ± 0.063 |
Mean activity (μmol·h−1·mg−1 protein) and standard deviations were calculated from triplicate assays of proteins bound to S-agarose from each of two independent preparations.