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RNA Gel Blot Analysis of the Induction of ERF1 Expression by Jasmonate (A), and Synergistic Effect of Ethylene and Jasmonate on the Expression of ERF1, b-CHI, and PDF1.2 (B).
Four-week-old wild-type Arabidopsis plants were treated with 100 ppm of ethylene (E), 50 μM jasmonic acid (JA), both (EJA), or not treated (A [air]) for the indicated times. Twenty micrograms of total RNA was loaded per lane, and blots were hybridized with the indicated probes and rDNA as a loading control.
