Figure 1.

Map-Based Cloning of the REF2 Gene.

(A) REF2 was mapped to a 60-kb mapping interval on BAC F18A5 containing 14 annotated genes. A contig of cosmids containing Landsberg genomic DNA was constructed that spanned the mapping interval (gray bars). Cosmids that complemented the sinapoylmalate and leaf glucosinolate phenotypes of ref2-1 overlap at CYP83A1 (black bars).

(B) RNA gel blot analysis of CYP83A1 expression in wild-type and ref2 rosettes. The graph shows levels of CYP83A1 expression normalized to cyclophilin (ROC3) expression. Error bars represent standard errors of triplicate assays. Col, Columbia wild type.