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. 2003 Jan;15(1):179–194. doi: 10.1105/tpc.006544

Figure 2.

Figure 2.

HPLC Analysis of Soluble Secondary Metabolites Accumulated in Leaves of Wild-Type, ref2-1, and a Representative ref2-1 Plant Transformed with a Cosmid Containing CYP83A1.

(A) Methanolic leaf extracts analyzed by HPLC. The elution of UV light–absorbing compounds was monitored at 320 nm. Col, Columbia wild type; SG, sinapoylglucose; SM, sinapoylmalate.

(B) Leaf desulfoglucosinolate fractions analyzed by HPLC. The elution of UV light–absorbing compounds was monitored at 229 nm. Peaks are magnified threefold for the first 15 min of the chromatograms to more clearly show differences in peak areas. Glucosinolates are identified as follows: 3MSOP, 3-methylsulfinylpropyl; 4MSOB, 4-methylsulfinylbutyl; Std, internal standard; I3M, indol-3-ylmethyl; 4MOI3M, 4-methoxyindol-3-ylmethyl.