Figure 4.

Analysis of Glucosinolate Levels in Wild-Type and ref2 Seeds.

(A) Seed desulfoglucosinolate fractions analyzed by HPLC. The elution of UV light–absorbing compounds was monitored at 320 nm. Glucosinolates are identified as follows: 4OHB, 4-hydroxybutyl; 4MSOB, 4-methylsulfinylbutyl; Std, internal standard; 5MSOP, 5-methylsulfinylpropyl; 7MSOH, 7-methylsulfinylheptyl; 4MTB, 4-methylthiobutyl; I3M, indol-3-ylmethyl; 8MSOO, 8-methylsulfinyloctyl; 5MTP, 5-methylthiopropyl; 3BZO, 3-benzoyloxypropyl; 4BZO, 4-benzoyloxybutyl; 7MTH, 7-methylthioheptyl; 8MTO, 8-methylthiooctyl.

(B) Quantification of levels of representative glucosinolates in wild-type and ref2 seeds. Error bars represent standard errors of at least triplicate assays. Col, Columbia wild type.