Figure 8.

Soluble Secondary Metabolites Accumulated in the Leaves of the Wild Type, fah1-2, fah1-4, ref2-1, and a fah1-2 ref2-1 Double Mutant.

(A) HPLC analysis of phenylpropanoids monitored at 320 nm. fah1-2 is a null allele of F5H, whereas fah1-4 is a leaky fah1 allele. Col, Columbia wild type; DM, double mutant; Flav, flavonoid; FM, feruloylmalate; SG, sinapoylglucose; SM, sinapoylmalate.

(B) Absorbance spectrum of the novel ferulate ester peak accumulated in fah1 mutants, and the spectrum of an authentic standard of ferulic acid (FA) for comparison.

(C) Mass spectra of sinapoylmalate from wild-type leaf extracts and feruloylmalate in fah1-2 leaf extracts.