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. Author manuscript; available in PMC: 2006 Apr 10.
Published in final edited form as: Cell Microbiol. 2006 Jan;8(1):107–119. doi: 10.1111/j.1462-5822.2005.00604.x

Fig. 1.

Fig. 1

YFP-CBD as a marker of Lm delivery into cytosol.

A and B. Epifluorescence images of (A) wild-type Lm and (B) hly Lm decorated with HGFP-CBD after incubation with the purified fluorescent protein.

C and D. Phase-contrast (left), SNARF-labelled Lm (middle) and YFP-CBD fluorescence (right) images. (C) Cells viewed at 10 min after infection contained bacteria in phase-bright vacuoles (arrows) which were not labelled with YFP-CBD. (D) Cells viewed 60 min after infection showed some bacteria labelled with YFP-CBD (arrows).

E. YFP fluorescence image of a RAW 264.7 macrophage showing YFP-CBD-positive cytosolic Lm (left) with corresponding labelling by CFP-actin (asterisks).