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Cytofluorometry of HeLa cells. (a and b) The untreated cells (a) and cells treated with Ph–TRITC (b) at pH 6.5 and 37°C are shown. (c–e) Cells treated (for 1 h) with 6 μM pHLIP–S–S–Ph–TRITC at pH 7.4 and 37°C (c), pH 6.5 and 37°C (d), and pH 6.5 and 4°C (e) are shown.
