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. Author manuscript; available in PMC: 2007 Jan 1.
Published in final edited form as: Am J Physiol Renal Physiol. 2005 Aug 9;290(1):F188–F195. doi: 10.1152/ajprenal.00285.2005

Figure 6.

Figure 6

Localization of transgene in kidney inner medulla by immunohistochemistry. A; Low power magnification shows expression of β-Gal transgene (red) is confined to the cells of the IMCD and is not apparent in the thin descending limbs of Henle’s loop (tL). Nuclei are stained blue. B; High power magnification demonstrates that β-Gal staining (red) is apparent in the cytoplasm of the IMCD principal cells. C; Staining is localized to the IMCD2 and IMCD 3 regions, with little staining of the base of the inner medulla, IMCD1. Colocalization studies with an anti-aquaporin 2 antibody demonstrate that β-Gal staining (D, red) and aquaporin 2 staining (E, green) are colocalized in IMCD principal celIs, F.