Supplementary Table 1.
Proteins identified with 2 or more unique peptides in AQP2-immunoisolated vesicles.
| Protein name | Accession | Gene name | # peptides | # unique peptides |
|---|---|---|---|---|
| Endosomal Trafficking | ||||
| annexin A2 | NP_063970 | Anxa2 | 51 | 18 |
| annexin A4 | NP_077069 | Anxa4 | 7 | 5 |
| annexin A5 | NP_037264 | Anxa5 | 9 | 6 |
| adaptor protein complex AP-1, beta 1 subunit or adaptor-related protein complex 2, beta 1 subunit | NP_058973 NP_542150 | Ap1B1 Ap2B1 | 9 | 5 |
| adaptor protein complex AP-2, alpha 2 subunit | NP_112270 | Ap2A2 | 7 | 7 |
| adaptor-related protein complex 2, mu 1 subunit | NP_446289 | Ap2M1 | 2 | 2 |
| ADP-ribosylation factor 1 or 2 or 3 | NP_071963 NP_031503 NP_543180 | Arf1 Arf2 Arf3 | 5 | 2 |
| ADP-ribosylation factor 6 | NP_077066 | Arf6 | 3 | 2 |
| similar to Ac39/physophilin | XP_214672 | Atp6V0D1 | 3 | 3 |
| clathrin, heavy polypeptide | NP_062172 | Cltc | 16 | 12 |
| similar to coatomer protein complex subunit alpha | XP_222899 | Copa | 3 | 3 |
| coatomer protein complex, subunit beta 2 (beta prime) | NP_068533 | Copb2 | 3 | 2 |
| similar to Myoferlin | XP_220031 | Fer1L3 | 2 | 2 |
| guanine nucleotide binding protein, alpha inhibiting 3 | NP_037238 | Gnai3 | 2 | 2 |
| guanine nucleotide-binding protein, beta-1 or -2 or –3 | NP_112249 NP_112299 NP_068630 | Gnb1 Gnb2 | 8 | 4 |
| cation-dependent mannose-6-phosphate receptor | NP_001007701 | M6Pr | 5 | 4 |
| myxovirus (influenza virus) resistance 1 or 2 | NP_775119 NP_599177 | Mx1 Mx2 | 4 | 2 |
| N-ethylmaleimide sensitive fusion protein attachment protein alpha | NP_542152 | Napa | 8 | 5 |
| Rab1 | NP_112352 | Rab1 | 15 | 3 |
| Rab2 | NP_113906 | Rab2 | 10 | 5 |
| Rab5B | XP_213824 | Rab5B | 2 | 2 |
| Rab5C | XP_213463 | Rab5C | 3 | 2 |
| Rab7 | NP_076440 | Rab7 | 12 | 6 |
| Rab10 | NP_059055 | Rab10 | 2 | 2 |
| Rab11A or 11B | NP_112414 NP_116006 | Rab11A Rab11B | 2 | 2 |
| Rab14 | NP_446041 | Rab14 | 7 | 3 |
| Rab18 | XP_225453 | Rab18 | 2 | 2 |
| Rab25 | XP_227404 | Rab25 | 3 | 3 |
| reticulon 4 | NP_114019 | Rtn4 | 13 | 4 |
| secretory carrier membrane protein 1 | XP_342175 | Scamp1 | 3 | 2 |
| secretory carrier membrane protein 2 | NP_076445 | Scamp2 | 4 | 2 |
| similar to gp250 precursor | XP_217115 | Sorl1 | 3 | 2 |
| sortilin 1 | XP_342318 | Sort1 | 3 | 2 |
| syntaxin 7 | NP_068641 | Stx7 | 11 | 4 |
| syntaxin 12 or 13 | NP_075228 AAC18967 | Stx12 | 5 | 4 |
| vesicle-associated membrane protein 2 | NP_036795 | Vamp2 | 10 | 3 |
| vesicle-associated membrane protein, associated protein A or B | NP_113819 NP_068619 | Vapa Vapb | 2 | 2 |
| valosin-containing protein | NP_446316 | Vcp | 6 | 5 |
| 14-3-3, zeta polypeptide | NP_037143 | Ywhaz | 3 | 3 |
| Endoplasmic Reticulum | ||||
| aldehyde dehydrogenase family 3, subfamily A2 | NP_113919 | Aldh3A2 | 2 | 2 |
| similar to hypothetical protein FLJ14511 | XP_232987 | Alg2 | 2 | 2 |
| sarcoplasmic/endoplasmic reticulum calcium ATPase 2 | P11507 | Atp2A2 | 9 | 5 |
| calreticulin | NP_071794 | Calr | 3 | 2 |
| calnexin | NP_742005 | Canx | 22 | 6 |
| carboxylesterase 2 | NP_598270 | Ces2 | 3 | 3 |
| cytochrome b-5 | NP_071581 | Cyb5 | 7 | 4 |
| similar to oligosaccharyltransferase | XP_233581 | Ddost | 9 | 5 |
| diaphorase 1 | NP_620232 | Dia1 | 3 | 3 |
| similar to RIKEN cDNA 1200007D18 | XP_213272 | Ergic32 | 4 | 3 |
| endoplasmic retuclum protein 29 | NP_446413 | Erp29 | 4 | 4 |
| similar to alpha glucosidase II, alpha subunit | XP_215144 | Ganab | 2 | 2 |
| glucosidase 1 | NP_113937 | Gcs1 | 4 | 4 |
| glucose regulated protein, 58 kDa | NP_059015 | Grp58 | 19 | 15 |
| similar to hexose-6-phosphate dehydrogenase precursor | XP_233688 | H6Pd | 2 | 2 |
| hydroxysteroid 11-beta dehydrogenase 1 | NP_058776 | Hsd11B1 | 10 | 4 |
| hydroxysteroid 11-beta dehydrogenase 2 | NP_058777 | Hsd11B2 | 9 | 7 |
| heat shock 70kD protein 5 | NP_037215 | Hspa5 | 11 | 9 |
| hypoxia up-regulated 1 | NP_620222 | Hyou1 | 9 | 6 |
| inositol 1,4,5-triphosphate receptor 1 | NP_001007236 | Itpr1 | 2 | 2 |
| similar to Vesicular integral-membrane protein VIP36 precursor | XP_214428 | Lman2 | 12 | 6 |
| prolyl 4-hydroxylase, beta polypeptide | NP_037130 | P4Hb | 7 | 6 |
| proteolipid protein 2 | NP_997484 | Plp2 | 14 | 2 |
| cyclophilin B | NP_071981 | Ppib | 14 | 4 |
| similar to alpha glucosidase II, beta subunit | XP_238534 | Prkcsh | 3 | 2 |
| coated vesicle membrane protein | NP_113910 | Rnp24 | 4 | 2 |
| serine (or cysteine) proteinase inhibitor, clade H, member 1 | NP_058869 | Serpinh1 | 3 | 3 |
| signal peptidase complex 18kD | NP_113911 | Spc18 | 3 | 2 |
| similar to RIKEN cDNA 5730406I15 | XP_214994 | Spc25 | 4 | 3 |
| signal sequence receptor, alpha | NP_001008891 | Ssr1 | 4 | 2 |
| signal sequence receptor 4 | NP_058895 | Ssr4 | 11 | 4 |
| integral membrane protein Tmp21-I | NP_445919 | Tmp21 | 8 | 5 |
| similar to Endoplasmin precursor | XP_343193 | Tra1 | 9 | 9 |
| thioredoxin domain containing 7 | NP_001004442 | Txndc7 | 13 | 8 |
| UDP-glucose ceramide glucosyltransferase-like 1 | NP_598280 | Ugcgl1 | 2 | 2 |
| Putative Cargo | ||||
| agrin | NP_786930 | Agrn | 4 | 2 |
| kidney aminopeptidase M | NP_112274 | Anpep | 3 | 2 |
| apolipoprotein E | NP_620183 | Apoe | 2 | 2 |
| aquaporin 1 | NP_036910 | Aqp1 | 6 | 3 |
| aquaporin 2 | NP_037041 | Aqp2 | 11 | 2 |
| ATPase, Na+/K+ transporting, alpha 1 polypeptide | NP_036636 | Atp1A1 | 39 | 12 |
| ATPase, Na+/K+ transporting, alpha 2 polypeptide | NP_036637 | Atp1A2 | 41 | 6 |
| ATPase, Na+/K+ transporting, beta 1 polypeptide | NP_037245 | Atp1B1 | 12 | 5 |
| similar to Cadherin-16 precursor | XP_226203 | Cdh16 | 18 | 7 |
| chloride channel K1 | NP_445779 | Clcnk1 | 2 | 2 |
| ceruloplasmin | NP_036664 | Cp | 8 | 6 |
| FXYD domain-containing ion transport regulator 2 isoform A or B | NP_663769 NP_059045 | Fxyd2 | 4 | 2 |
| insulin-like growth factor 2 receptor | NP_036888 | Igf2R | 4 | 4 |
| integrin alpha 2 | XP_345157 | Itga2 | 4 | 3 |
| similar to VLA-3 alpha subunit | XP_340885 | Itga3 | 2 | 2 |
| similar to integrin alpha v subunit | XP_230950 | Itgav | 5 | 4 |
| integrin beta 1 | NP_058718 | Itgb1 | 3 | 3 |
| aminopeptidase Vp165 | NP_598258 | Lnpep | 18 | 10 |
| Lutheran blood group | NP_113940 | Lu | 8 | 3 |
| membrane bound C2 domain containing protein | NP_058945 | Mbc2 | 12 | 9 |
| chloride ion pump-associated 55 kDa protein | NP_659553 | Pcyox1 | 2 | 2 |
| Rap1A or 1B | NP_002875 NP_599173 | Rap1A Rap1B | 8 | 4 |
| similar to band 7 protein | XP_219858 | Spfh1 | 2 | 2 |
| similar to CDNA sequence BC036333 | XP_214372 | Spfh2 | 2 | 2 |
| tumor-associated calcium signal transducer 1 | NP_612550 | Tacstd1 | 8 | 4 |
| transmembrane emp24 protein transport domain containing 4 | NP_872353 | Tmed4 | 2 | 2 |
| transmembrane protein 4 | NP_064337 | Tmem4 | 3 | 2 |
| Ribosomal | ||||
| acidic ribosomal protein P0 | NP_071797 | Arbp | 13 | 7 |
| similar to ribosome-binding protein p34 | NP_001008281 | Mgc105677 | 5 | 2 |
| similar to 60S ribosomal protein L11 | XP_342950 | Rpl11 | 10 | 2 |
| similar to 60S ribosomal protein L12 | XP_216039 | Rpl12 | 4 | 3 |
| ribosomal protein L13 | NP_112363 | Rpl13 | 10 | 2 |
| ribosomal protein L27 | NP_071959 | Rpl27 | 2 | 2 |
| ribosomal protein L30 | NP_073190 | Rpl30 | 5 | 2 |
| ribosomal protein L31 | NP_071951 | Rpl31 | 3 | 2 |
| ribosomal protein L6 | NP_446423 | Rpl6 | 4 | 2 |
| 60S ribosomal protein L7a | P62425 | Rpl7A | 4 | 3 |
| ribosomal protein L8 | XP_343279 | Rpl8 | 4 | 2 |
| 60S acidic ribosomal protein P2 | P02401 | Rplp2 | 6 | 3 |
| ribosomal protein S11 | NP_112372 | Rps11 | 3 | 2 |
| ribosomal protein S13 | NP_569116 | Rps13 | 4 | 3 |
| ribosomal protein S15a | NP_446434 | Rps15A | 3 | 2 |
| similar to 40S ribosomal protein S16 | XP_341816 | Rps16 | 4 | 3 |
| 40S ribosomal protein S18 | P62271 | Rps18 | 5 | 2 |
| similar to 40S ribosomal protein S2 | XP_214903 | Rps2 | 4 | 2 |
| ribosomal protein S26 | NP_037356 | Rps26 | 8 | 2 |
| 40S ribosomal protein S3 | P62909 | Rps3 | 3 | 3 |
| ribosomal protein S4, X-linked | NP_001007601 | Rps4X | 2 | 2 |
| ribosomal protein S6 | NP_058856 | Rps6 | 3 | 2 |
| similar to 40S ribosomal protein S7 | XP_345691 | Rps7 | 3 | 2 |
| ribosomal protein S8 | NP_113894 | Rps8 | 9 | 4 |
| ribosomal protein S9 | NP_112370 | Rps9 | 3 | 2 |
| Nuclear | ||||
| similar to KIAA0788 protein | XP_215831 | Ascc3L1 | 3 | 2 |
| similar to Probable RNA-dependent helicase p72 | XP_235480 | Ddx17 | 12 | 7 |
| similar to Probable RNA-dependent helicase p68 | NP_001007614 | Ddx5 | 5 | 4 |
| H1 histone family, member 4 | NP_579819 | H1F4 | 2 | 2 |
| histone 2a | NP_068612 | H2A | 29 | 2 |
| H2A histone family, member Z | NP_073165 | H2Afz | 11 | 2 |
| H3 histone, family 3B | NP_446437 | H3F3B | 2 | 2 |
| histone 1, H2bl | NP_072173 | Hist1H2Bl | 5 | 3 |
| germinal histone H4 gene | NP_073177 | Hist1H4B | 18 | 4 |
| heterogeneous nuclear ribonucleoprotein A3 | NP_937765 | Hnrnpa3 | 4 | 2 |
| similar to heterogeneous nuclear ribonucleoprotein H3 isoform a | XP_342132 | Hnrph3 | 7 | 3 |
| poly(rC) binding protein 1 | NP_035995 | Pcbp1 | 3 | 2 |
| neuronal differentiation-related gene | NP_647549 | Prp19 | 3 | 3 |
| similar to splicing factor Prp8 | XP_213385 | Prpf8 | 2 | 2 |
| similar to heterogeneous nuclear ribonucleoprotein G | XP_229192 | Rbmx | 9 | 3 |
| similar to RIKEN cDNA 1810061H24 | XP_214697 | Sf3B3 | 10 | 8 |
| similar to PTB-associated splicing factor | XP_342921 | Sfpq | 3 | 3 |
| similar to 116 kDa U5 small nuclear ribonucleoprotein component | XP_213492 | Snrp116 | 2 | 2 |
| similar to Small nuclear ribonucleoprotein Sm D2 | XP_214847 | Snrpd2 | 4 | 3 |
| small nuclear ribonucleoparticle-associated protein | NP_112379 | Snrpn | 3 | 2 |
| Cytoskeletal-Motor | ||||
| cytoplasmic beta-actin or gamma-actin | NP_112406 P63259 | Actb Actg1 | 75 | 17 |
| actin, gamma 2 | NP_037025 | Actg2 | 3 | 3 |
| similar to RIKEN cDNA 2010015J01 | XP_237111 | Arpc5L | 2 | 2 |
| beta-catenin | NP_445809 | Catnb | 2 | 2 |
| gelsolin | NP_001004080 | Gsn | 3 | 3 |
| type II keratin Kb1 | NP_001008802 | Kb1 | 9 | 4 |
| similar to cytokeratin | XP_215513 | Krt1-18 | 3 | 2 |
| keratin complex 1, acidic, gene 19 | NP_955792 | Krt1-19 | 7 | 6 |
| keratin complex 2, basic, gene 8 | NP_955402 | Krt2-8 | 3 | 2 |
| myosin regulatory light chain | NP_059039 | Mrlcb | 16 | 5 |
| myosin heavy chain 10, non-muscle | NP_113708 | Myh10 | 20 | 14 |
| myosin, heavy polypeptide 9 | NP_037326 | Myh9 | 69 | 31 |
| Myosin light polypeptide 6 | Q64119 | Myl6 | 17 | 6 |
| similar to Myosin VI | XP_236444 | Myo6 | 2 | 2 |
| unconventional myosin Myr2 I heavy chain | NP_075580 | Myr2 | 19 | 14 |
| beta-spectrin 3 | NP_062040 | Spnb3 | 5 | 5 |
| tubulin, alpha 1 | NP_071634 | Tuba1 | 6 | 4 |
| tubulin, beta 5 | NP_775125 | Tubb5 | 6 | 5 |
| Unclassified | ||||
| similar to RIKEN cDNA 5730469M10 | XP_341407 | 8 | 5 | |
| similar to DKFZP586A0522 protein | XP_235659 | 4 | 2 | |
| similar to Hypothetical protein KIAA0152 | XP_222228 | 5 | 3 | |
| similar to RIKEN cDNA 1500009M05 | XP_215704 | 4 | 2 | |
| aldehyde reductase 1 | NP_036630 | Aldr1 | 7 | 3 |
| crystallin, alpha B | NP_037067 | Cryab | 4 | 4 |
| hydroxysteroid (17-beta) dehydrogenase 12 | NP_114455 | Hsd17B12 | 2 | 2 |
| heat shock protein 8 | NP_077327 | Hspa8 | 5 | 4 |
| heat shock 90kDa protein 1, beta | NP_001004082 | Hspcb | 4 | 4 |
| similar to pM5 protein | XP_341862 | Nomo1 | 4 | 3 |
| similar to CG8014-PA | XP_236584 | Rme-8 | 6 | 5 |
| Mitochondrial | ||||
| ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit | NP_599191 | Atp5B | 3 | 3 |
| voltage-dependent anion channel 1 | NP_112643 | Vdac1 | 4 | 3 |
| voltage-dependent anion channel 2 | NP_112644 | Vdac2 | 4 | 3 |
| Extracellular (secreted) | ||||
| collagen, type XVIII, alpha 1 | XP_241632 | Col18A1 | 27 | 4 |
| fibronectin 1 | NP_062016 | Fn1 | 3 | 3 |
| similar to Basement membrane-specific heparan sulfate proteoglycan core protein precursor | XP_233606 | Hspg2 | 3 | 2 |