. Author manuscript; available in PMC: 2006 Apr 12.

Published in final edited form as: J Biol Chem. 2000 Sep 8;275(36):28128–28138. doi: 10.1074/jbc.M004488200

Table III. Stereospecificities of RDHs in the retina and RPE.

UV-treated RPE microsomes, ³H-labeled retinoids, and dinucleotides were prepared as described under “Materials and Methods.” The assay was carried out using phase partition method as described under “Materials and Methods.” The concentration of dinucleotide inhibitors was 1 mm.

Enzyme source	Retinoids	Dinucleotides	Activity	Stereospecificity retinoid/dinucleotide
			nmol/min/mg
ROS	all-trans-retinal	pro-R-[4-³H]NADPH	ND^a
	all-trans-retinal	pro-S-[4-³H]NADPH	19.35 ± 0.35	pro-S
	pro-R-[15-³H]all-trans-retinol	NADP	3.06 ± 0.01	pro-R
	pro-S-[15-³H]all-trans-retinol	NADP	ND
	all-trans-retinal	pro-R-[4-³H]NADH	ND
	all-trans-retinal	pro-S-[4-³H]NADH	ND
prRDH	all-trans-retinal	pro-R-[4-³H]NADPH	ND
	all-trans-retinal	pro-S-[4-³H]NADPH	1.69 ± 0.05	pro-S
	pro-R-[15-³H]all-trans-retinol	NADP	1.46 ± 0.06	pro-R
	pro-S-[15-³H]all-trans-retinol	NADP	ND
	all-trans-retinal	pro-R-[4-³H]NADH	ND
	all-trans-retinal	pro-S-[4-³H]NADH	ND
RPE	11-cis-retinal	pro-R-[4-³H]NADH	0.1 ± 0.01
	11-cis-retinal	pro-S-[4-³H]NADH	4.95 ± 0.09	pro-S
	11-cis-retinal	pro-R-[4-³H]NADPH	ND
	11-cis-retinal	pro-S-[4-³H]NADPH	0.84 ± 0.06	pro-S
	pro-R-[15-³H]11-cis-retinol	NAD	0.074 ± 0.002
	pro-S-[15-³H]11-cis-retinol	NAD	0.63 ± 0.02	pro-S
	pro-R-[15-³H]11-cis-retinol	NADP	0.034 ± 0.003
	pro-S-[15-³H]11-cis-retinol	NADP	0.066 ± 0.001	pro-S
RPE + NADH	11-cis-retinal	pro-R-[4-³H]NADPH	ND
	11-cis-retinal	pro-S-[4-³H]NADPH	0.48 ± 0.01	pro-S
RPE + NADPH	11-cis-retinal	pro-R-[4-³H]NADH	0.08 ± 0.01
	11-cis-retinal	pro-S-[4-³H]NADH	3.07 ± 0.01	pro-S
11-cis-RDH	11-cis-retinal	pro-R-[4-³H]NADH	ND
	11-cis-retinal	pro-S-[4-³H]NADH	387 ± 28	pro-S
	pro-R-[15-³H]11-cis-retinol	NAD	ND
	pro-S-[15-³H]11-cis-retinol	NAD	112 ± 2	pro-S

ND, not detectable.