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. 2006 Apr 13;116(5):1230–1242. doi: 10.1172/JCI26772

Figure 2. Reduced inflammatory reaction to trauma in the absence of PlGF.

Figure 2

(A) Top panel: PFD3 WT tibia stained by H&E to visualize the fracture localization, the box corresponding to the area shown in panels below. Bottom panels: Morphology of WT and PlGF–/– calluses at PFD3 shown by H&E staining and inflammatory cell accumulation in the fracture environment shown by CD45 staining on subsequent sections. Cortical bone fragments (asterisks) are lined in red. Arrowheads indicate fracture site. m, muscle; f, fixator. Scale bar: 500 μm. (B) Detailed view of areas 1 and 2 boxed in A, showing more abundant accumulation of CD45-positive cells in WT than in PlGF–/– calluses, both on the broken cortex fragments (top panel) and in the marrow near the fracture site (bottom panel). Scale bar: 200 μm. (C) Hematopoietic colony-forming cell assay of BM and spleen derived from WT and PlGF–/– mice, showing the number of colonies (hematopoietic progenitor CFU) counted at day 9 of culture. E, erythroid; M, monocyte/macrophage; G, granulocyte. **P < 0.01.