FIG. 8.

Effect of inhibitors of mitochondrial metabolism on H₂O₂ production in 3T3-L1 adipocytes under high glucose conditions. Fully differentiated 3T3-L1 adipocytes were maintained in either 5 mM glucose with 20 mM mannitol or 25 mM glucose as shown, prior to treatment with the indicated mitochondrial inhibitor (CCCP, OHCA, TTFA) for 1 h prioto measurement of H₂O₂ production by DCF-DA loading and confocal fluorescence microscopy. The data from a dose-response with each of the tested inhibitors is shown. ^* < 0.05 and ^**p < 0.001 (CCCP); ^**p < 0.001 (OHCA); ^* = 0.002 and ^**p < 0.001 (TTFA), compared with the control samples in 25 mM glucose without inhibitor.