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Effect of copy number of orfX-containing fragments on pigment production in S. lividans TK21 cultured in liquid YEME medium for 5 days. (A) ▪, pIJ941; □, pLS4 (pIJ941 containing 3.6-kb EcoRI-PstI fragment of S1). (B) ▪, pIJ61; □, pMS4 (pIJ61 containing 2.7-kb BamHI-PstI fragment of S1). (C) ▪, pIJ702; □, pPRA4 (pIJ702 containing 3.6-kb SacI-PstI fragment of S2). Act, actinorhodin; Und, undecylprodigiosin.
