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Schematic presentation of construction of orfX mutant. (A) Restriction map and mutagenesis of orfX by insertion of gentamicin resistance gene (aac′). (B) Confirmation of insertion of aac′ gene into orfX by PCR analysis with internal primers S36F5 and S36R2. Wt, wild-type strain; Mut, orfX mutant. Sizes are shown in kilobases.
