TABLE 2.
Primers used in sequencing and PCR
| Primer | Nucleotide sequence (5′ to 3′) | Position |
|---|---|---|
| Forward | ||
| S18F1 | ACCTCCACTTCCGGTCGCTGA | 128-148 |
| S18F2 | ATCCATCTCATTCTGGGCGTC | 380-400 |
| S33F1 | TCGTGAGCAGCAGGTTGAGC | 2024-2043 |
| S33F2 | AAACCATCGGCGTTGACGGT | 2455-2474 |
| S33F3 | TCCCACACGTCGCCGATCAA | 2805-2824 |
| S33F4 | ACGCCTGGTACGCGGCGTTC | 3229-3248 |
| S36F1 | CCGGCAATGACTCGTCCCAC | 5239-5258 |
| S36F2 | ACGGGCGTGCGGTGTCGAT | 5536-5554 |
| S36F3 | TCTACACGGACCGGGTCATC | 5871-5890 |
| S36F4 | CCGGGCACTTCGAGCACGTC | 6192-6211 |
| Reverse | ||
| S18R1 | AAGTTCCGCAAGGACGGCGTA | 876-856 |
| S18R2 | AACGGACGGCGTTCCTCGCC | 1220-1201 |
| S18R3 | GGCAGTGAGCTCTTCGAGAA | 1596-1577 |
| S33R1 | GACCTGGCGCACATCGGCAA | 3751-3732 |
| S33R2 | AATCCGCTGTGGATCGAGGC | 4131-4112 |
| S33R3 | TCGACCCGGCAGTACCTGTG | 4548-4529 |
| S33R4 | TACGCCGCATTGCGGACCGT | 4992-4973 |
| S36R1 | TCCGCAAGCCGGCCGACGCGAA | 7456-7435 |
| S36R2 | AAGCTGGGTCTGATCGTCGA | 7817-7798 |
| PCR | ||
| Aac1 | AAGCTCGAGAAGGCCCGATCCTT GGAGCCC | pHP45Ω45aac′ |
| Aac2 | AAGCTCGAGCTCTTGAGTTAAGC CGCGCCG | pHP45Ω45aac′ |
| S36F5 | AAGCCTTACCGTTTCACCCC | 6827-6846 |
| S36R2 | AAGCTGGGTCTGATCGTCGA | 7817-7798 |