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. 2006 Apr 14;2(4):e30. doi: 10.1371/journal.ppat.0020030

Figure 1. Overexpression of Flagellin by MogR-Negative L. monocytogenes at RT Results in a Chaining Phenotype.

Figure 1

(A) Western blot analysis of FlaA levels in cellular fractions of wild-type and ΔmogR strains. L. monocytogenes cultures were grown at RT or 37 °C for approximately 24 h. Secreted, surface-localized, and cytoplasmic FlaA protein was detected by Western blot using a FlaA-specific antibody.

(B) L. monocytogenes strains wild-type, ΔflaA, ΔmogR, and ΔmogR ΔflaA were grown at RT or 37 °C for approximately 24 h, stained for flagella, and analyzed by phase-contrast microscopy. Open arrowheads indicate flagella. The percentage of chaining events within a given population is shown below each panel. A chaining event was defined as a chain of three or more bacteria. A total of 300 bacterial events were counted for each population. The mean and standard deviation of three independent experiments are given.